NAR Molecular Biology Database Collection entry number 1008
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-5065, USA

Database Description

qPrimerDepot is a primer database for RT-PCR analysis of greater than 99% of human and mouse RefSeq genes. These primers sets are designed to be used under uniform annealing temperatures to facilitate their application in large scale high throughput assays. For intron-bearing genes, over 90% of the primer sets are designed to produce amplicons bridging exon:exon junctions of intron-bearing genes. Thus, preserving the signal to noise ratio against genomic contamination. The database is available on line at http://primerdepot.nci.nih.gov/ and http://mouseprimerdepot.nci.nih.gov/.

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