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MiCroKit


NAR Molecular Biology Database Collection entry number 1022
Ren, J.1,2, Liu, Z.2, Gao, X.2, Jin, C.2, Ye, M.3, Zou, H.3, Wen, L.2, Zhang, Z.4, Yao, X.2*, and Xue, Y.1,2*
1Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
2Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
3National Chromatographic R and A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China
4Banting and Best Department of Medical Research and Department of Molecular Genetics, Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada

Database Description

During cell division/mitosis, a specific subset of proteins is spatially and temporally assembled into protein super-complexes in three distinct regions, i.e. centrosome/spindle pole, kinetochore/centromere and midbody/cleavage furrow/phragmoplast/bud neck, and modulates the cell division process faithfully. Although many experimental efforts were carried out to investigate the characteristics of these proteins, no integrated database was available. Here, we present the MiCroKit database (http://microkit.biocuckoo.org) of proteins that localize in midbody, centrosome, and/or kinetochore. We collected into the MiCroKit database experimentally verified microkit proteins from the scientific literature that have unambiguous supporting evidence for subcellular localization under fluorescence microscopy. The current version of MiCroKit 3.0 provides detailed information for 1,489 microkit proteins from seven model organisms, including S. cerevisiae, S. pombe, C. elegans, D. melanogaster, X. laevis, M. musculus and H. sapiens. Moreover, orthology information was provided for these microkit proteins, and could be a useful resource for further experimental identification. The online service of MiCroKit database was implemented in PHP + MySQL + JavaScript, while the local packages were developed in JAVA 1.5 (J2SE 5.0).

Acknowledgements

The authors thank Dr. Michael Galperin for his constructive and helpful suggestions. This work was supported by grants from the National Basic Research Program (973 project) (2002CB713700, 2006CB933300, 2007CB914503), National Natural Science Foundation of China (90919001, 30700138, 30830036, 30721002, 30871236, 20605022 and 90713017), Chinese Academy of Sciences (KSCX2-YW-R-139, KSCX1-YW-R65, INFO-115-C01-SDB4-36), the China High Technology Research Program (2008ZX1002-020), the Cultivation Fund of the Ministry of Education of China (NO706035), and National Science Foundation for Post-doctoral Scientists (20080430100). Z.Z. acknowledges funding support from Canadian Institutes of Health Research.

References

1. Xue Y, Zhou F, Fu C, Jin C, Pei S, Xu Y, Yao X. MiCroKit: An Integrated Database of Midbody, Centrosome and Kinetochore. Submitted.


Go to the abstract in the NAR 2010 Database Issue.
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