NAR Molecular Biology Database Collection entry number 1022
Huang, Zhengnan; Ma, Lili; Wang, Yongbo; Pan, Zhicheng; Ren, Jian; Liu, Zexian; Xue, Yu
1Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
22State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, China

Database Description

We reported an updated database of MiCroKiTS 4.0 (http://microkit.biocuckoo.org) for proteins temporally and spatially localized in distinct subcellular positions including midbody, centrosome, kinetochore, telomere and mitotic spindle during cell division/mitosis. The database was updated from our previously developed database of MiCroKit 3.0 (1), which contained 1,489 proteins mostly forming super-complexes at midbody, centrosome and kinetochore from seven eukaryotes. Since the telomere and spindle apparatus are critical for cell division, the proteins localized at the two positions were also integrated. From the scientific literature, we curated 1,872 experimentally identified proteins which at least locate in one of the five positions from eight species. Then the ortholog detection was performed to identify potential MiCroKiTS proteins from 144 eukaryotic organisms, which contains 66, 45 and 33 species of animals, fungi and plants, respectively. In total, 87,983 unique proteins with corresponding localization information were integrated into the database (2). The primary references of experimentally identified localizations were provided and the fluorescence microscope figures for the localizations of human proteins were shown. The orthologous relations between predicted and experimental localizations were also present. Taken together, we anticipate the database can serve as a useful resource for further analyzing the molecular mechanisms during cell division.


This work was supported by grants from the National Basic Research Program (973 project) (2013CB933900, 2012CB910101, and 2012CB911201), Natural Science Foundation of China (31171263, 81272578, J1103514 and 31071154), International Science & Technology Cooperation Program of China (2014DFB30020), and China Postdoctoral Science Foundation (2014M550392).


1. Ren J., Liu Z., Gao X., Jin C., Ye M., Zou H., Wen L., Zhang Z., Xue Y., Yao X. (2010) MiCroKit 3.0: an integrated database of midbody, centrosome and kinetochore. Nucleic Acids Res., 38, D155-160.
2. Huang Z., Ma L., Wang Y., Pan Z., Ren J., Liu Z., Xue Y. (2014) MiCroKiTS 4.0: a database of midbody, centrosome, kinetochore, telomere and spindle. (Accepted by NAR).

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