NAR Molecular Biology Database Collection entry number 108
Mitchell, Alex; Scheremetjew, Maxim; Denise, Hubert; Potter, Simon; Tarkowska, Aleksandra; Qureshi, Matloob; Salazar, Gustavo; Pesseat, Sebastien; Boland, Miguel; Hunter, Fiona; Ten Hoopen, Petra; Alako, Blaise; Amid, Clara; Wilkinson, Darren; Curtis, Thomas; Cochrane, Guy; Finn, Robert
EBI metagenomics (http://www.ebi.ac.uk/metagenomics) provides a free to use platform for the analysis and archiving of sequence data derived from the microbial populations found in a particular environment. Over the past two years, EBI metagenomics has increased the number of datasets analysed 10-fold. In addition to increased throughput, the underlying analysis pipeline has been overhauled to include both new or updated tools and reference databases. Of particular note is a new workflow for taxonomic assignments that has been extended to include assignments based on both the large and small subunit RNA marker genes and to encompass all cellular micro-organisms. We also describe the addition of metagenomic assembly as a new analysis service. Our pilot studies have produced over 2400 assemblies from datasets in the public domain. From these assemblies, we have produced a searchable, non-redundant protein database of over 50 million sequences. To provide improved access to the data stored within the resource, we have developed a programmatic interface that provides access to the analysis results and associated sample metadata. Finally, we have integrated the results of a series of statistical analyses that provide estimations of diversity and sample comparisons.
Category: Genomics Databases (non-vertebrate)
Subcategory: Fungal genome databases
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