NAR Molecular Biology Database Collection entry number 225
Hoogland C.
Swiss Institute of Bioinformatics, 1 rue Michel-Servet, CH-1211 Genève 4, Switzerland

Database Description

The SWISS-2DPAGE database has been established in 1993 and is maintained collaboratively by the Central Clinical Chemistry Laboratory of the Geneva University Hopital and the Swiss Institute of Bioinformatics (SIB). The current release (Octobre, 2003) of the SWISS-2DPAGE database contains about 1200 entries in 36 reference maps from human samples (Liver, Plasma, HepG2, Red blood cells, Lymphocyte, Lymphoma, HepG2 Secreted Proteins, Cerebrospinal Fluid, Macrophage Like Cell Line, Erythroleukemia Cell, Platelet, Kidney, Promyelocytic Leukemia Cells, Colorectal epithelia cells, Colorectal adenocarcinoma cell line (DL-1), Soluble nuclear proteins and matrix from liver tissue, SDS-PAGE and 2-D PAGE of nucleolar proteins from Human HeLa cells), from mouse samples (Liver, Gastrocnemius muscle, Pancreatic islet cells, White and Brown adipose tissue, Soluble nuclear proteins and matrix from liver tissue), Arabidopsis thaliana, Dictyostelium discoideum, Escherichia coli (for 7 pI ranges: 3.5-10, 4-5, 4.5-5.5, 5-6, 5.5-6.7, 6-9, 6-11; and 2D-DIGE for pI range 4.5-6.5), Saccharomyces cerevisiae and Staphylococcus aureus. On these maps about 4000 spots have been identified by gel matching (39%), immunoblotting (17%), microsequencing (13%), amino acid composition (4.5%), comigration (2%), peptide mass fingerprinting (27%) and tandem mass spectrometry (13%). Some of the spots have been identified by several methods, which explains that total exceeds 100%. Through the ExPASy server, the SWISS-2DPAGE database can be queried by keywords (protein description, protein name, gene name, species, author, full text, protein spot serial number) or graphically by clicking on a spot. One can also compute the estimated location on each SWISS-2DPAGE reference maps for any protein sequence of the SWISS-PROT database or user-entered amino acids sequence.

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