NAR Molecular Biology Database Collection entry number 319
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA

Database Description

UniSTS is a comprehensive database of sequence tagged sites (STSs), each of which is defined by a distinct pair of primer sequences designed for use in a PCR reaction. As of November 2004, it includes about 460,000 STSs derived from 225 organisms. STSs are widely used in genome mapping and researchers may directly submit STS maps to the database. UniSTS is tightly integrated with the NCBI Map Viewer displays of STS maps. In addition, STSs are regularly incorporated from other databases, including dbSTS, RHdb, and several model organism databases. When the genomic sequence becomes available for an organism, it is of interest to find the positions of all the mapped STSs within the sequence, both to validate the assembly and to integrate STS maps that may not have shared many common markers. This information is provided by UniSTS through pre-computed results of matching PCR primers to the sequence (“electronic PCR”). In a similar fashion, connections are established to other types of genomic and transcript sequences. Finally, UniSTS entries are augmented with links to helpful information in other databases, such as PubMed, GenBank, UniGene, dbSNP, and OMIM.

Recent Developments

UniSTS was a database of sequence tagged sites (STSs), derived from STS-based maps and other experiments. All data from this resource have been moved to the Probe database. You can retrieve all UniSTS records by searching the probe database using the search term "unists[properties]". Additionally, legacy data remain on the NCBI FTP Site in the UniSTS Repository (ftp://ftp.ncbi.nih.gov/pub/ProbeDB/legacy_unists).

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