Potsdam 2009
Day 1...
MHR Tweet: Antral follicle development achieved in In-vitro cow model in 10 days
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic.
Quote from Session 3 - Evelyn Telfer
Talk: Improvements in media and culture conditions in human follicle culture.
"We have been able to achieve antral follicular development using multi-step culture within 10 days. So we are almost the whole way to getting embryos in an in vitro model (cow) and there is no reason why we cannot do the same in human".
For further details contact Evelyn Telfer: Evelyn.Telfer@ed.ac.uk
MHR Tweet: The prospects of long-term oocyte culture depend upon replicating in vivo environment in vitro
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic
Quote from Session 3 - Helen Picton
Talk: Carbohydrate and energy metabolism during follicle growth and oocyte maturation: what can be learned for IVM and follicle culture?
"Metabolism is the key to oocyte health. Normal egg cells are more efficient than chromosomally deficient cells. Amino acid production profiles in vitro predict oocyte developmental potential.
"The prospects of long-term culture from early stages to maturity depend upon replicating in vivo environment in vitro."
For further details contact Helen Picton: h.m.picton@leeds.ac.uk
MHR Tweet: A new approach and a new philosophy to IVM: induced IVM based on biphasic culture
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic
Quote from Session 3 - Robert Gilchrist
Talk: Improving the performance of IVM in clinics and in animal breeding.
“A new approach and a new philosophy to IVM: induced IVM based on biphasic culture: pre-IVM (forskolin +IBMX) followed by extended-IVM (FSH+PDE inhibitor). This is being successfully applied in mouse, cow and human”.
"Standard IVM is an artifact. IVM is currently half as efficient asIVF at best. Our key objective to overcome this gap and develop IVM into a technique for patients that really need it. Our approach is to use attenuated IVM using cAMP modulators".
For further details contact Robert Gilchrist: robert.gilchrist@adelaide.edu.au
MHR Tweet: The human ovary is the best model for the human
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic
Quote from Richard Anderson, Centre for Reproductive Biology, University Edinburgh
Talk: "The human ovary is the best model for the human by and large. A new research area for us is the potential for germ cell regulation by prostaglandin-E2. We are trying to tease out what the factors might be and how they might impact germ cell meiosis".
For further details contact Richard Anderson: r.a.anderson@hrsu.mrc.ac.uk
Day 2...
MHR Tweet:”How do primordial germ cells get to the gonads? Man similar to mouse in that…”
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic.
Quote from Session 6: Anne Grete Byskov
Talk: From primordial germ cells to oocytes
“How do primordial germ cells get to the gonads? In mouse and man, the answer is passive translocation from yolk sac to hindgut during lateral folding followed by transport to the gonadal ridge via mesenteric neurons”
For further details contact Anne Grete Byskov: agb.lrb@rh.dk
MHR Tweet: “The Epidermal Growth Factor network plays a critical role in ovulation”
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic.
Quote from Session 6: Marco Conti
Talk: EGF-network in maturation induction and oocyte quality
“There is overwhelming pharmacological and genetic evidence that the
Epidermal Growth Factor network plays a critical role in ovulation.
Ovulation-inducing LH surge suppresses granulosa cell cGMP levels
prior to GVDB – this too is dependent on EGF signaling.”
“After 10 yr work we are much closer to understanding pathways
involved in meiotic arrest and maturation of the oocyte”.
For further details contact Marco Conti: marco.conti@stanford.edu
MHR Tweet “ERK1/2 control the molecular switch by which LH reprogrammes granulosa cells and cumulus cells in preovulatory follicles”
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic.
Quote from Session 6 : Joanne Richards
Talk: Immune-like mechanisms in ovulation
“ERK1/2 control the molecular switch by which LH reprogrammes
granulosa cells and cumulus cells in preovulatory follicles”
For further details contact Joanne Richards: joanner@bcm.tmc.edu
MHR Tweet “Loss of Zfp57 causes loss of DNA methylation imprints in the oocyte”
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic.
Quote from Session 7: Xiajun (John) Li
Talk: A maternal zygotic effect gene maintains genomic imprinting in embryos
“Loss of Zfp57 causes loss of DNA methylation imprints. Ablating
maternal Zfp57 in the oocyte can cause maternal-zygotic embryonic
lethality through perturbing the imprinting status at the Dlk1-Gtl2
domain”.
For further details contact Xiajun (John) Li: xiajun.li@mssm.edu
MHR Tweet: “Understanding epigenetic reprogramming in the embryo is crucial for human infertility treatment”
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic.
Quote from Session 7: Thomas Haaf
Talk: Methylation reprogramming dynamics and defects in gametogenesis and embryogenesis: implications for reproductive medicine
“A better understanding of epigenetic reprogramming in the fertilized
egg and embryo and identification of possible genetic modifiers and
environmental factors are crucial for improving human infertility
treatment”
“The male embryo may be more vulnerable to epigenetic reprogramming
defects during gametogenesis and early embryogenesis”
For further details contact Thomas Haaf: haaf@humgen.klinik.uni-mainz.de
MHR Tweet: "Rac-GTP is required for chromosome congression, spindle stability and polar body extrusion but not actin cap formation during oocyte meiosis…”
6th Workshop on Mammalian folliculogenesis and oogenesis: from basic science to the clinic.
Quote from Session 8: John Caroll
Talk: Dynamic localisation of Protein kinase A in immature and mature oocytes and preimplantation embryos: marker for developmental capacity?
"Rac-GTP is required for chromosome congression, spindle stability and
polar body extrusion but not actin cap formation during oocyte meiosis…”
For further details contact John Carroll: j.carroll@ucl.ac.uk
