NAR Molecular Biology Database Collection entry number 6
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20894, USA
Expressed sequence tag (EST) analysis is being applied to a growing number of organisms. UniGene provides an organized view of these transcribed sequences by collapsing them into groups that correspond to genes and then connecting these entries to other classes of information that may shed light on gene expression and function. Traditionally, this grouping has been based on all-against-all sequence alignment among the transcript sequences. For the few organisms for which a genomic sequence is available, improved results have recently been obtained by using the genome as a scaffold for grouping transcripts. By taking genomic position into account, it is possible to bring together gene fragments with very short overlaps and to separate near-identical gene family members that might have been inappropriately merged. Tools provided on the UniGene web site allow researchers to browse cDNA libraries by developmental stage or tissue of origin, view surrogate expression profiles based on EST counts, and find groups of genes with similar expression patterns.
Category: Human and other Vertebrate Genomes
Subcategory: Human ORFs
Go to the abstract in the NAR 2004 Database Issue.
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