NAR Molecular Biology Database Collection entry number 783
Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Faculty of Medicine, Monash University, Clayton, Victoria, Australia
The majority of human proteins expressed in bacteria are insoluble and thus require renaturation. Identifying the optimal refolding conditions and methodology is therefore rate limiting. In order to address this problem, we have catalogued the methods employed in the refolding of proteins in a web-accessible relational database, REFOLD. Entries are heavily annotated such that the database can be searched via multiple parameters. The database will have two roles: First, as a data repository and experimental resource, new data can be rapidly deposited, validated and made available to the refolding and wider scientific community. Much valuable refolding data goes unpublished (for example, in cases where a protein cannot be refolded for structural studies), thus we aim to exploit this untapped resource by encouraging deposition in REFOLD. The database will thus become a powerful experimental tool for the optimization of protein expression, refolding and purification, bypassing the relatively slow and inefficient examination of the literature. Second, REFOLD will become a valuable resource for theoreticians looking for relationships between refolding success and protein characteristics such as charge and hydrophobicity. Data mining may uncover general predictive rules that could facilitate the refolding of novel proteins.
Category: Protein sequence databases
Subcategory: Protein properties
Go to the abstract in the NAR 2006 Database Issue.
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